1-Hexyl-3-Methylimidazolium Chloride-Potassium Carbonate Aqueous Two Phase System: Equilibrium Characteristics and BSA Partitioning Behavior

Abstract

The partitioning behavior of the model protein (bovine serum albumin) was investigated in ionic liquid (1-hexyl-3-methylimidazolium chloride) -salt (potassium carbonate) based aqueous two phase system (ATPS). The phase diagram with binodal curve and tie lines for the selected ATPS was developed at different temperatures and analyzed through effective excluded volume (EEV) and Othmer-Tobias and Bancroft equations, respectively. The influence of various process parameters like the ionic liquid and salt concentration, system temperature, tie line length, phase volume ratio, and neutral salt addition on partition coefficient/extraction efficiency of BSA protein was evaluated. 2014 Copyright Taylor & Francis Group, LLC.